A novel reverse phase high-performance liquid chromatography method for standardization of Orthosiphon stamineus leaf extracts
Noor Hafizoh Saidan1, Abdalrahim F.A. Aisha1, Mohd Shahrul Ridzuan Hamil1, Amin Malik Shah Abdul Majid2, Zhari Ismail1★★ Corresponding author
- 1Departments of Pharmaceutical Chemistry
- 2Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pulau Pinang, Malaysia
CORRESPONDENCE
Zhari Ismail
*Address for correspondence: Prof. Zhari Ismail, Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pulau Pinang, Malaysia.
Revised: 04-06-2014.
Volume 7, Issue 1 · pp. 23–31 · PUBLISHED January-March 2015 · DOI: 10.4103/0974-8490.147195
ABSTRACT
Background Orthosiphon stamineus Benth. (Lamiaceae) is a traditional medicinal plant which has been used in treating various ailments such as kidney diseases, bladder inflammation, arthritis and diabetes. The leaves contain high concentration of phenolic compounds, thus, rosmarinic acid (RA), 3’-hydroxy-5, 6, 7, 4’-tetramethoxyflavone (TMF), sinensetin (SIN) and eupatorin (EUP) were chosen as a marker compounds for standardization of various O. stamineus leaf extracts. Objective The aim was to develop and validate a new high-performance liquid chromatography (HPLC) method for quantification of 4 marker compounds (RA, TMF, SIN, EUP) in various O. stamineus leaf extracts. Materials and Methods The method was developed and validated using RP-HPLC-diode-array detection at 320 nm for accuracy, precision and limits of detection and was applied for quantification of it markers in five different extracts prepared in solvents with increasing polarity, using a gradient mobile phase 0.1% formic acid: Acetonitrile at a flow rate of 1 ml/min on reverse phase acclaim polar advantage II C18 column (3 mm, 3 × 150 mm) with 18 min separation time. Results The developed method provided satisfactory precision, and the accuracy of this method was in the range of 90.2% to 105.5%. All of 4 compounds showed good linearity at R2 > 0.999. Conclusion The developed method is a simple, cost effective with shorter run time (18 min) in comparison to previous methods (30 min) and utilization of environmental-friendly solvents system. Therefore, this method has the potential to replace currently used methods in the routine standardization work of O. stamineus extracts, raw materials and its commercial products.
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Saidan, N. H., Aisha, A. F., Hamil, M. S. R., Majid, A. M. S. A., & Ismail, Z. (2015). A novel reverse phase high-performance liquid chromatography method for standardization of Orthosiphon stamineus leaf extracts. Pharmacognosy Research, 7(1), 23–31. https://doi.org/10.4103/0974-8490.147195