Preclinical screening of phyllanthus amarus ethanolic extract for its analgesic and antimicrobial activity
S. Sham Bhat1, K. Sundeep Hegde1, Sharath Chandrashekhar1, S. N. Rao2, Shyamjith Manikkoth2★★ Corresponding author
- 1Departments of Paedodontics and Preventive Dentistry
- 2Pharmacology, Yenepoya University, Mangalore, Karnataka, India
CORRESPONDENCE
Shyamjith Manikkoth
*Address for correspondence: Dr. Shyamjith Manikkoth, Department of Pharmacology, Yenepoya University, Mangalore, Karnataka, India.
Revised: 08-01-2015.
Volume 7, Issue 4 · pp. 378–392 · PUBLISHED October-December 2015 · DOI: 10.4103/0974-8490.159577
ABSTRACT
Background To discover a new agent which possesses dual property of analgesic and antimicrobial activity, thereby reducing the burden of polypharmacy. Phyllanthus amarus was screened for its analgesic and antimicrobial activities. Objectives The objective was to evaluate the analgesic and antimicrobial activity, of P. amarus ethanolic extract (PAEE). Materials and Methods The ethanolic extract of P. amarus was prepared using Soxhlet apparatus. An in vivo study using Swiss albino mice was done to screen the central and peripheral analgesic activity of P. amarus extract. The extract was administered at a dose of 100 mg/kg body weight orally. The peripheral analgesic activity was assessed using acetic acid induced writhing test. The central analgesic activity was assessed using Eddy’s hot plate apparatus. An in vitro study was carried out to study the antimicrobial activity of the above extract using selected species of Streptococcus mutans, and S. salivarius. The antimicrobial activities were determined using the agar well method. Results The ethanolic extract of P. amarus showed significant (P < 0.05) peripheral and central analgesic activity. In vitro antimicrobial screening indicated that the ethanolic extract had shown a zone of inhibition against S. mutans and S. salivarius in the agar wells. Conclusion This study showed that PAEE exhibited significant analgesic and antimicrobial activities.
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Bhat, S. S., Hegde, K. S., Chandrashekhar, S., Rao, S. N., & Manikkoth, S. (2015). Preclinical screening of phyllanthus amarus ethanolic extract for its analgesic and antimicrobial activity. Pharmacognosy Research, 7(4), 378–392. https://doi.org/10.4103/0974-8490.159577