jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Pharmacy PracticeNOW IN
Article

Development of a new spectrophotometric method for the determination of flavonoids in herb of red clover (Trifolium pratense L) and zigzag clover (Trifolium medium L)

Alexander Vladimirovich Nikulin1, Fedor Valerievich Radus1, Ludmila Ilyinichna Fedorina1, Yulia Alexandrovna Efimova1, Mikhail Alexandrovich Lazov1, Elena Valeryevna Mironova1, Darya Aleksandrovna Trashchenkova2, Ekaterina Anatolevna Dorovskikh3, Ekaterina Vyacheslavovna Sergunova3, Natal'ya Vladimirovna Bobkova3, Elena Nikolaevna Kostikova3, Sergey Viktorovich Lutsenko4, Valeriya Alexandrovna Savina4, Olga Georgievna Potanina5, Rimma Aleksandrovna Abramovich5, Tatiana Yurievna Kovaleva3

  1. 1I.P. Alimarin Department of Analytical Chemistry of MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies).
  2. 2Pharmacology Department, First Sechenov Moscow State Medical University (Sechenov University).
  3. 3Department of Pharmaceutical Natural Sciences, Institute of Pharmacy, Sechenov First Moscow State Medical University (Sechenov University).
  4. 4Department of Biotechnology, Sechenov First Moscow State Medical University (Sechenov University).
  5. 5Pharmacognosy and Industrial Pharmacy Department, Faculty of Medicine, Lomonosov Moscow State University.

Received: 15-04-2025; Accepted: 02-07-2025.

Volume 23, Issue 4 · pp. 3384 · PUBLISHED 12 November 2025 · DOI: 10.18549/PharmPract.2025.4.3384

View on PP original site ↗

ABSTRACT

Objective: Medicinal plants containing flavonoids are attracting increasing interest of researchers due to their complex therapeutic effects on the human body. Such medicinal plants include representatives of the genus Clover - red clover (Trifolium pratense L.) and zigzag clover (Trifolium medium L.). Methods: However, spectrophotometric techniques for the determination of the total flavonoids have numerous disadvantages, which prompted the development of a new analytical technique. As a result of our research, such a technique using gallium (III) nitrate as a staining reagent instead of the traditionally used aluminium chloride was developed, and a standard for recalculation (luteolin) was substantiated. Results: It has been shown that the new methodology is capable of giving satisfactory results, is characterised by excellent metrological characteristics, meets the requirements of SP RF XV ed. and the leading pharmacopoeias of the world (EP, USP, JP). Conclusion: The proposed methodology can be recommended for use not only for scientific purposes, but also in the field of standardisation of plant raw materials at pharmaceutical industries specialising in the production of medicinal preparations of plant origin.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Nikulin, A. V., Radus, F. V., Fedorina, L. I., Efimova, Y. A., Lazov, M. A., Mironova, E. V., Trashchenkova, D. A., Dorovskikh, E. A., Sergunova, E. V., Bobkova, N. V., Kostikova, E. N., Lutsenko, S. V., Savina, V. A., Potanina, O. G., Abramovich, R. A., & Kovaleva, T. Y. (2025). Development of a new spectrophotometric method for the determination of flavonoids in herb of red clover (Trifolium pratense L) and zigzag clover (Trifolium medium L). Pharmacy Practice, 23(4), 3384. https://doi.org/10.18549/PharmPract.2025.4.3384