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Article

In silico Comparative Analysis of the Pathogenicity and Molecular Characteristics of Toxic Shock Syndrome Caused by Staphylococcus aureus and Streptococcus pyogenes

Relika Balatcopo Maglinao1★, Jillian Mikaela Velasco Jamito1, Lyndon Manalastas Kalaw1, Elara Ysabelle Nava Laud1, Joan Mari Magsombol Lim1, Graziele Ann Lanting Manimtim1, Ania Lisa Galisanao Marcelo1, Kiana Ajiah Capili Nefulda1, Earl Adriane Abella Cano1★ Corresponding author

  1. 1Department of Medical Technology, Institute of Health Sciences and Nursing, Far Eastern University-Manila, Nicanor Reyes St., Sampaloc, Manila, Metro Manila, INDIA.

CORRESPONDENCE

Relika Balatcopo Maglinao

Department of Medical Technology, Institute of Health Sciences and Nursing, Far Eastern University-Manila, Nicanor Reyes St., Sampaloc, Manila, Metro Manila, INDIA.

2020002421@feu.edu.ph

Received: 23-06-2024; Revised: 09-06-2024; Accepted: 11-07-2024.

Volume 13, Issue 2 · pp. 472–480 · PUBLISHED · DOI: 10.5530/ajbls.2024.13.59

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ABSTRACT

Background:Toxic Shock Syndrome (TSS) is a medical condition caused by Staphylococcus aureusand Streptococcus pyogenes, affecting T-Cell Receptors (TCRs) and Major Histocompatibility Complex (MHC) in both healthy and immunocompromised individuals. Aim:The study identifies the pathogenicity of S. aureusand S. pyogenesby analyzing the molecular characteristics of the causative proteins in TSS development, TSST-1, or Toxic Shock Syndrome Toxin, in S. aureusand the Streptococcal Pyrogenic Exotoxins SpeA and SpeC in S. pyogenes. Materials and Methods:Through in silicoanalysis, the protein sequences were obtained from the NCBI GenBank and ran for physicochemical profiling with ProtParam and PsortB, the secondary structure determination with PROTEUS2 and Phyre2. Predicted Antigenic Peptide software served to compare the antigenic capacity. Homology modeling through SWISS-MODEL was validated using QMEANDisCo and GMQE scores, then DeepGOWeb for its function identification. Results:The analysis revealed SpeA as the most antigenic, with antigen-presenting cells and molecular function in toxin and binding activities. TSST-1 and SpeC also showed antigenicity levels, with TSST-1 being the most antigenic due to its hydrophilic nature. SpeC, with its lowest cellular process and intracellular structure, is primarily deficient in inducing TSS in patients. Conclusion:The study’s data will aid in the intervention of TSS patients, particularly immunocompromised individuals, benefiting them and public health. Antigenicity Hydropathicity Immunocompromised Toxic Shock Syndrome

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    Maglinao, R. B., Jamito, J. M. V., Kalaw, L. M., Laud, E. Y. N., Lim, J. M. M., Manimtim, G. A. L., Marcelo, A. L. G., Nefulda, K. A. C., & Cano, E. A. A. (). In silico Comparative Analysis of the Pathogenicity and Molecular Characteristics of Toxic Shock Syndrome Caused by Staphylococcus aureus and Streptococcus pyogenes. Asian Journal of Biological and Life sciences, 13(2), 472–480. https://doi.org/10.5530/ajbls.2024.13.59