Investigations on Apoptotic Activities of Cherry Laurel Extracts in HCT-116 Human Colon Carcinoma Cells
Bilal Çakir2, İbrahim Gülseren3★★ Corresponding author
- 1İstanbul Sabahattin Zaim University, Halal Foods R&D Center, Halkalı, Küçükçekmece, İstanbul, TURKEY.
- 2Department of Biochemistry, Marmara University, Institute of Health Sciences, Faculty of Pharmacy, Başıbüyük, Maltepe, İstanbul.
- 3Department of Food Engineering, İstanbul Sabahattin Zaim University, Halkalı, Küçükçekmece, İstanbul, TURKEY.
CORRESPONDENCE
İbrahim Gülseren
Department of Food Engineering, İstanbul Sabahattin Zaim University, Halkalı, Küçükçekmece, İstanbul, TURKEY.
Received: 13-12-2018; Revised: 06-02-2019; Accepted: 27-03-2019.
Volume 53, Issue 3S · pp. s264–s272 · PUBLISHED 2019 · DOI: 10.5530/ijper.53.3s.96
ABSTRACT
Background: Cherry laurel fruits are characterized by their dark color and rich phenolic contents. Previously, phenolic extracts from cherry laurel varieties were shown to demonstrate anti-proliferative activities against cancerous cell lines. Aim: To characterize the phenolic compound content of cherry laurel varieties since their characterization is incomplete and determine their apoptotic potential. Methods: The phenolic compounds of fruit extracts from 3 different cherry laurel varieties were analyzed using an LC-MS/MS method. Using flow cytometry, apoptotic activities of cherry laurel extracts were studied against HCT-116 human colon carcinoma cells. Results: 14 different phenolic compounds were qualitatively determined, whereas for 5 compounds quantitation was possible. To the best of our knowledge, the presence of epicatechin, epigallocatechin, quercetin, quercetin-3-glucoside, procyanidin B2, delphinidine-3-O-rutinoside, delphinidine-3-Oglucoside, cyanidine-3-O-glucoside and cyanidine-3-O-rutinoside is being reported for the first time for these extracts. Although some dependence on phenolic profile and concentration was obvious, in most cases, a significant extent of apoptotic induction (40-60%) was observed against HCT-116 cells. Conclusion: Findings on anti-proliferative and apoptotic activity verified the anti-carcinogenic capabilities of cherry laurel extracts.
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Çakir, B., & Gülseren, B. (2019). Investigations on Apoptotic Activities of Cherry Laurel Extracts in HCT-116 Human Colon Carcinoma Cells. Indian Journal of Pharmaceutical Education and Research, 53(3S), s264–s272. https://doi.org/10.5530/ijper.53.3s.96