jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Pharmacological Modulation of Flavivirus Methyltransferase Activity of Dengue Virus (DEV)

Afaf Salim Alwabli1★, Sana Ghazi Alattas1, Alawiah Mohammad Alhebshi1, Nidal Mohammed Zabermawi1, Fatima Kaneez2, Esam Ibraheem Azhar3, Naseer Alkenani1, Khalid Al-Ghmady1, Ishtiaq Qadri1★ Corresponding author

  1. 1Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, SAUDI ARABIA.
  2. 2Department of Applied Biochemistry, Faculty of Science, University of Jeddah, Jeddah, SAUDI ARABIA.
  3. 3Special Infectious Agents Unit, King Fahd Center for Medical Research, King Abdulaziz University, Jeddah, SAUDI ARABIA.

CORRESPONDENCE

Afaf Salim Alwabli

Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, SAUDI ARABIA.

aalwabili@stu.kau.edu.sa

Received: 27-04-2019; Revised: 17-05-2019; Accepted: 29-06-2019.

Volume 53, Issue 3S · pp. s433–s436 · PUBLISHED 2019 · DOI: 10.5530/ijper.53.3s.116

View on IJPER original site ↗

ABSTRACT

Background: The occurrence of dengue fever plagues has expanded significantly in the course of the most recent couple of decades. Be that as it may, no immunization or antiviral treatments are accessible. In this manner, the requirement for protected and successful antiviral medications have turned out to be essential. Aim: Methyltransferases, with their altered enzymatic action, have been found associated with several diseases or infections. It has been observed that the dengue virus infection is correlated with the hyperactivity of methyltransferase. In the current study, we proposed to investigate the inhibitory effects of commercially available compounds on the methyltransferase activity. Materials and Methods: Evaluation of methyltransferase activity of the purified enzyme and their inhibitors was carried out using Methyltransferase Activity Assay Kit (Colorimetric). Results: Among the screened components, we identify two effective compounds that revealed significant effect against flavivirus methyltransferase activity. Further, time-dependent inhibition profiles for both compounds revealed that preincubation of fractions of Azacitidine and Zebularine for different time windows showed time-dependent inactivation of methyltransferase as the degree of inhibition increased with time. Conclusion: We conclude that both Azacitidine and Zebularine may be emerging as possible suitable drugs for the treatment of dengue virus infection.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Alwabli, A. S., Alattas, S. G., Alhebshi, A. M., Zabermawi, N. M., Kaneez, F., Azhar, E. I., Alkenani, N., Al-Ghmady, K., & Qadri, I. (2019). Pharmacological Modulation of Flavivirus Methyltransferase Activity of Dengue Virus (DEV). Indian Journal of Pharmaceutical Education and Research, 53(3S), s433–s436. https://doi.org/10.5530/ijper.53.3s.116