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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Asarone and Metformin Modulates the Oxidant- Antioxidant Imbalance on Experimentally Induced Hepatocellular Carcinoma during Diabetic Condition

Bhrigu Kumar Das1,2, Pramod Channabasappa Gadad1,2★★ Corresponding author

  1. 1Department of Pharmacology, KLE College of Pharmacy (A Constituent Unit of KLE Academy of Higher Education and Research, Belagavi), Vidyanagar, Hubballi, Karnataka, INDIA.
  2. 2Off-campus Basic and Applied Sciences Research Centre of KLE Academy of Higher Education and Research at KLE College of Pharmacy, Vidyanagar, Hubballi, Karnataka, INDIA.

CORRESPONDENCE

Pramod Channabasappa Gadad

Department of Pharmacology, KLE College of Pharmacy (A Constituent Unit of KLE Academy of Higher Education and Research, Belagavi), Vidyanagar, Hubballi, Karnataka, INDIA.

gadadpramod@gmail.com

Received: 04-05-2020; Revised: 14-07-2020; Accepted: 09-09-2020.

Volume 54, Issue 4 · pp. 1039–1045 · PUBLISHED 2020 · DOI: 10.5530/ijper.54.4.198

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ABSTRACT

Aim: The aim of the present study was planned to investigate the efficacy of asarone and metformin HCl as an antioxidant on experimentally induced diabetic-hepatocellular carcinoma (HCC) condition in male Wistar rats. Methods: Diabetes was induced in experimental rats by single intraperitoneal injection of streptozotocin (STZ; 55 mg/kg b.w.). Following two weeks of STZ injection, the diabetic-HCC condition was simulated in the rats with a single intraperitoneal dose of diethylnitrosamine (DEN; 200 mg/ kg b.w.). The α-and β-asarone combination in the ratio of 1:1 (50 μg/kg b.w.) and metformin HCl (250 mg/kg b.w.) treatment were orally given to the diabetic-HCC rats up to 18-weeks. At the end of the experimental period, rats were sacrificed after the withdrawal of blood for biochemical and liver samples were isolated for the antioxidant and histopathological analysis. Results: The elevated levels of liver function test such as alkaline phosphatase, bilirubin and decreased levels of total protein, albumin and globulin indicated the hepatic damage in the diabetic-HCC rats. Further, the STZ+DEN-induced oxidative stress was confirmed by the elevated levels of lipid peroxidation (LPO) and decreased levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and Vitamin-C. Treatment with asarone and metformin significantly ameliorated the STZ+DEN-induced hepatic damage and oxidative stress. Histopathological evidence also showed recovery of the hepatic architecture in diabetic- HCC rats treated with asarone and metformin. Conclusion: Asarone and metformin exhibit good hepatoprotective and antioxidant potential against STZ+DEN-induced HCC in rats.

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    Das, B. K., & Gadad, P. C. (2020). Asarone and Metformin Modulates the Oxidant- Antioxidant Imbalance on Experimentally Induced Hepatocellular Carcinoma during Diabetic Condition. Indian Journal of Pharmaceutical Education and Research, 54(4), 1039–1045. https://doi.org/10.5530/ijper.54.4.198