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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Preparation, Characterization and Optimization of Repurposed Valproic Acid Loaded Carboxymethyl Chitosan Nanoparticles by Box–Behnken Design for Alzheimer Management

Ayush Jaiswal1, Senthil Venkatachalam1★, Anindita De2, Bhavesh Namdev1, Ravikumar Ramaswamy3, Jawahar Natarajan1★ Corresponding author

  1. 1Department of Pharmaceutics, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, INDIA.
  2. 2Assistant Professor, College of Pharmacy, JSS Academy of Technical Education, Noida, INDIA.
  3. 3Department of Advanced Materials and Engineering, Hanseo University, 360, Daegok-ri, Seosan-si 356 706, Chungcheongnam-do, SOUTH KOREA.

CORRESPONDENCE

Senthil Venkatachalam

Department of Pharmaceutics, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, INDIA.

senthil.v@jssuni.edu.in

Received: 16-04-2020; Revised: 14-07-2020; Accepted: 20-01-2021.

Volume 55, Issue 1S · pp. S75–S86 · PUBLISHED 2021 · DOI: 10.5530/ijper.55.1s.39

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ABSTRACT

Aim/Background: Alzheimer’s disease (AD) is one of the most ominous diseases, leads dementia but highest unmet till today. The new strategy needed via repositioning due to the limitation of existing therapies. Valproic acid is one of the first line drugs for epilepsy and bipolar disorder showed neuroprotective potential by decreased Aβ production in AD. Methods: The objective of this research was to prepare the repurposed VPA loaded polymeric nanoparticles using carboxymethyl chitosan for management of AD through the intranasal route. Polymeric nanoparticle (PNPs) was prepared by ionic gelation method and optimization was done using Box-Behnken design (BBD) model. Results: Characterization of optimized PNPs was done for Particle size which was found to be 384± 1.45 nm with drug release of 82.39% ± 3.1 after 48 hr. In vitro cytotoxicity using cell line, SH-SY5Y and IC50 value of optimized formulation was found to be 42.96 μg/ml. Ex vivo histopathological study was performed using goat nasal mucosa for the Nasalcilio toxicity studies which showed no inflammation and toxicity for VPA-CMC PNPs and indicate the safe formulation. Conclusion: The outcome of the research is a positive direction to reuse the VPA for the management of AD in the near future through the intranasal route.

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    Jaiswal, A., Venkatachalam, S., De, A., Namdev, B., Ramaswamy, R., & Natarajan, J. (2021). Preparation, Characterization and Optimization of Repurposed Valproic Acid Loaded Carboxymethyl Chitosan Nanoparticles by Box–Behnken Design for Alzheimer Management. Indian Journal of Pharmaceutical Education and Research, 55(1S), S75–S86. https://doi.org/10.5530/ijper.55.1s.39