jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Proof-of-concept for Site-specific Delivery of Mesalamine Nanoparticles for Effective Therapy in Colitis

Nagaraja Sreeharsha1,3★, Rashmi Narayan2, Joshi Vijaya2, Anroop Nair1★ Corresponding author

  1. 1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, SAUDI ARABIA.
  2. 2Department of Pharmaceutics, Government College of Pharmacy, Bangalore, Karnataka, INDIA.
  3. 3Department of Pharmaceutics, Vidya Siri College of Pharmacy, Off. Sarjapura Road, Bangalore, Karnataka, INDIA.

CORRESPONDENCE

Nagaraja Sreeharsha

Dr. Sree Harsha Nagaraja,Associate Professor, Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa-31982, SAUDI ARABIA.

sharsha@kfu.edu.sa

Received: 15-04-2021; Revised: 28-05-2021; Accepted: 30-05-2021.

Volume 55, Issue 2S · pp. s448–s456 · PUBLISHED 2021 · DOI: 10.5530/ijper.55.2s.116

View on IJPER original site ↗

ABSTRACT

Background: Mesalamine loaded polymeric nanoparticles were prepared using three different polymers namely, Eudragit RS100, PLGA (50:50), or Eudragit RLPO, with an aim of targeted delivery to the inflamed colon. Materials and Methods: Nanoparticles were prepared by modified emulsification solvent evaporation and characterized for various physicochemical characteristics viz., size, size distribution, mesalamine entrapment, and in-vitro release. Results: Amongst the various formulations prepared, formulation F5 mesalamine nanoparticles made with Eudragit RS100 showed drug entrapment of 72.09% with comparative discrete nearing spherical particle size of 200 nm. In-vivo targeting potential of nanoparticles to the inflamed tissue was evaluated in acetic acid-induced colitis rat model and efficacy was compared with pristine mesalamine powder. Biochemical estimations were carried in colonic tissue homogenate to check the oxidative damage. The myeloperoxidase activity and lipid peroxides were significantly decreased and glutathione content increased after the oral administration of mesalamine nanoparticles compared to pure drug mesalamine. Conclusion: This delivery system enabled the drug to accumulate in the inflamed tissue with higher efficiency than the pure drug thus nanoparticulate system was efficient in mitigating the experimental colitis.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Sreeharsha, N., Narayan, R., Vijaya, J., & Nair, A. (2021). Proof-of-concept for Site-specific Delivery of Mesalamine Nanoparticles for Effective Therapy in Colitis. Indian Journal of Pharmaceutical Education and Research, 55(2S), s448–s456. https://doi.org/10.5530/ijper.55.2s.116