Methylmercury, Mn2+ and Pb2+ Exposure Promotes Premature Proliferation/Differentiation of Human Neural Stem Cells in Different Ways
Wenyan Ying1,2, Tongbing Qi1,2, Dongzi Shi1,2, Jing Zhang2, Ganghui Ye2, Xuewen Tian1, Qinglu Wang1,2★★ Corresponding author
- 1College of Sports and Health, Shandong Sport University, Jinan, CHINA.
- 2Key Laboratory of Biomedical Engineering and Technology of Shandong High School, Qilu Medical University, Zibo, CHINA.
CORRESPONDENCE
Qinglu Wang
College of Sports and Health, Shandong Sport University, Jinan-330013, CHINA.
Received: 21-12-2021; Revised: 24-06-2022; Accepted: 29-09-2022.
Volume 57, Issue 1 · pp. 155–160 · PUBLISHED 2023 · DOI: 10.5530/001954641252
ABSTRACT
Background: Methylmercury (MeHg), manganese ions (Mn2+), and lead ions (Pb2+) are ubiquitous environmental pollutants and may be neurotoxic especially during fetal development. We decided to explore the toxic mechanisms of MeHg (organic heavy metals), Mn2+ (inorganic heavy metals) and Mn2+ on the proliferation and differentiation of human neural stem cells (hNSCs). Materials and Methods: The proliferation and apoptosis of hNSCs were analyzed via CCK-8 method and flow cytometry under MeHg, Mn2+ and Pb2+, respectively. RNA-seq was used for analyzing proliferation/differentiation mechanism of MeHg, Mn2+ and Pb2+ stressing hNSCs. Results: Our experiment found that when hNSCs were exposed to below 0.5 nM MeHg, 5μM Mn2+ and 10 μM Pb2+, cell proliferation and differentiation were promoted. Apoptosis rates increased significantly when hNSCS were exposed to exceed 0.5 nM MeHg, 5μM Mn2+ and 10 μM Pb2+. RNA-seq results showed that metal ions altered the genes expression level and signaling pathways of hNSC differentiation and proliferation, but the regulatory mechanisms of MeHg, Mn2+ and Pb2+ were different. Conclusion: Our findings indicated that very low-dose metal exposure may deplete hNSC pool by making prematurely differentiated neurons increase, which may be the real cause of long-term nervous system disruption in adulthood, rather than higher metal doses will cause more direct toxicity during infant development.
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Ying, W., Qi, T., Shi, D., Zhang, J., Ye, G., Tian, X., & Wang, Q. (2023). Methylmercury, Mn2+ and Pb2+ Exposure Promotes Premature Proliferation/Differentiation of Human Neural Stem Cells in Different Ways. Indian Journal of Pharmaceutical Education and Research, 57(1), 155–160. https://doi.org/10.5530/001954641252