jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Combination of N-acetyl Cysteine and Thymoquinone Alleviates Hepatocellular Toxicities by Radiation and CCl4 Intoxication in SD Rats

Shu Zhang1, Shaohua Chen2, Jingyi Wang2, Qingyu Sun3★, Tahani Awad Alahmadi4, Nandakumar Natarajan5★ Corresponding author

  1. 1Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, CHINA.
  2. 2Department of General Surgery, Affiliated Hospital of Chifeng University, Chifeng, Inner Mongolia Autonomous Region, CHINA.
  3. 3Department of General Surgery, Medical College of Nantong University, The Affiliated Changzhou No. 2 People’s Hospital of Nanjing Medical University, Changzhou, Jiangsu Province, CHINA.
  4. 4Department of Pediatrics, College of Medicine and King Khalid University Hospital, King Saud University, Medical City, Riyadh, SAUDI ARABIA.
  5. 5Department of Cellular and Molecular Biology, Center for Biomedical Research, The University of Texas at Tyler Health Science Center, Tyler, Texas, USA.

CORRESPONDENCE

Qingyu Sun

Department of General Surgery, Medical College of Nantong University, The Affiliated Changzhou No. 2 People’s Hospital of Nanjing Medical University, Changzhou, Jiangsu Province, CHINA.

sunqingyu138@outlook.com

Received: 28-09-2022; Revised: 22-12-2022; Accepted: 06-02-2023.

Volume 57, Issue 3 · pp. 802–812 · PUBLISHED 2023 · DOI: 10.5530/ijper.57.3.98

View on IJPER original site ↗

ABSTRACT

Radiation and chemicals were the major clinical toxicants known to cause cellular damage during prognostic interventions in vivo. Cellular and molecular damages in the liver were the major causes for the hepatocellular injury due to various toxicities. Though many antioxidants alleviate various types of hepatotoxicities, protection exerted by the combination of N-acetyl cysteine (NA) and Thymoquinone (TQ) in the combined toxicities of radiation and carbon tetrachloride (CCLR) in Sprague-Dawley (SD) rats were unknown. Current research was focused on the protective efficacy of combination of NA with optimized dose of TQ (NATQ) in radiation/CCl4 (CCLR)-induced hepatotoxicities. At the end of the experimental period (6 weeks), body weight, liver weight, serum and liver tissues were analyzed for marker enzymes (AST, ALT, LDH), oxidative stress level (MDA, GSSG), antioxidant status (GSH, Vitamin E, Vitamin C), activities of enzymatic antioxidants (SOD, GPx, CAT, GST), liver histopathology and studies for the hepatic levels of NfkB, IL-6, TNF-α, MMP-3, MMP-12, Nrf2 and HO-1 were done. Significant (p≤0.05) toxicological alterations in the above parameters were recovered to normal in the treatment of NATQ combinations in SD rats. In conclusion, we provide evidence of protective efficacy of NATQ combination in alleviating the hepatotoxicities produced by CCLR in SD rats.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Zhang, S., Chen, S., Wang, J., Sun, Q., Alahmadi, T. A., & Natarajan, N. (2023). Combination of N-acetyl Cysteine and Thymoquinone Alleviates Hepatocellular Toxicities by Radiation and CCl4 Intoxication in SD Rats. Indian Journal of Pharmaceutical Education and Research, 57(3), 802–812. https://doi.org/10.5530/ijper.57.3.98