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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

The Effect of Sophoridine on NSCLC by Down Regulation of mTOR and NOTCH1

Mohammad Raghibul Hasan1, Ali Hazazi2,3, Ahad Amer Alsaiari4, Fawaz M. Almufarriji1, Amirah Albaqami5, Osama Abdulaziz4, Marwa K Darwish1,6, Atul Kumar7, Kapil Dev7, Mohammed Ageeli Hakami1★★ Corresponding author

  1. 1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah, Riyadh, SAUDI ARABIA.
  2. 2Department of Pathology and Laboratory Medicine, Security Forces Hospital Program, Riyadh, SAUDI ARABIA.
  3. 3College of Medicine, Alfaisal University, Riyadh, SAUDI ARABIA.
  4. 4Department of Clinical Laboratory Science, College of Applied Medical Science, Taif University, Taif, SAUDI ARABIA.
  5. 5Department of Clinical Laboratory Sciences, Turabah University College, Taif University, Taif, SAUDI ARABIA.
  6. 6Department of Chemistry (Biochemistry Branch), Faculty of Science, Suez University, Suez, EGYPT.
  7. 7Department of Biotechnology, Jamia Millia Islamia, Jamia Nagar, New Delhi, INDIA.

CORRESPONDENCE

Mohammed Ageeli Hakami

Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah-19257, Riyadh, SAUDI ARABIA.

m.hakami@su.edu.sa

ORCID: 0000-0002-1747-1277

Received: 13-07-2024; Revised: 27-08-2024; Accepted: 13-09-2024.

Volume 58, Issue 4 · pp. 1373–1379 · PUBLISHED Jul-Sep 2024 · DOI: 10.5530/ijper.58.4.147

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ABSTRACT

Introduction Sophoridine, an alkaloid compound which is isolated from various plant species like Sophoraalopecuroides and Sophora flavescens. It identified to have potential pharmacological properties, including anti-inflammatory, anticancer and antiviral activities. The effect of the sophoridine on mTOR and NOTCH1 pathways that are critical in NSCLC is not well understood. Materials and Methods In order to assess the virtual interaction of mTOR and NOTCH1 with sophoridine, we employed molecular docking. We also performed an in vitro analysis using the NSCLC cell line (A549) using MTT, RT-PCR, western blot and wound healing assays. Results and Discussion We report strong interaction of sophoridine with mTOR and it exhibited cytotoxic effect on NSCLC cells, causing downregulation of the mTOR and NOTCH1 genes. We used a wound-healing assay to assess its impact on cell migration in NSCLC cells and our findings confirmed that sophoridine significantly (p = 0.0265) inhibits cell migration in A549 cells. Conclusion Our study indicates sophoridine to be potential anti-cancer drug in NSCLC cell via inhibition of mTOR and NOTCH1 axis.

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    Hasan, M. R., Hazazi, A., Alsaiari, A. A., Almufarriji, F. M., Albaqami, A., Abdulaziz, O., Darwish, M. K., Kumar, A., Dev, K., & Hakami, M. A. (2024). The Effect of Sophoridine on NSCLC by Down Regulation of mTOR and NOTCH1. Indian Journal of Pharmaceutical Education and Research, 58(4), 1373–1379. https://doi.org/10.5530/ijper.58.4.147