jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Expression of Helicobacter pylori CagA in Solanum melongena L-A Cost Effective Strategy for Vaccine Development

Mohammad Javad Mehran1★, Rambod Barzigar1, Basaralu Yadurappa Sathish Kumar1,2, Nanjundappa Haraprasad3, Bashasab Fakrudin4, Sayan Paul5, Sudhakar Malla6, Mansoor Bolideei7★ Corresponding author

  1. 1Department of Biotechnology, JSS Research Foundation, SJCE Technical Campus, University of Mysore, Mysore, Karnataka, INDIA.
  2. 2Department of Biotechnology, JSS College, Ooty Road, Mysore, Karnataka, INDIA.
  3. 3Department of Biotechnology, JSS Science and Technology University, SJCE, Technical Campus, Mysore, Karnataka, INDIA.
  4. 4Department of Biotechnology and Crop Improvement, College of Horticulture, University of Horticulture Sciences Campus, GKVK Post, Bangalore, Karnataka, INDIA.
  5. 5Department of Biochemistry and Molecular Biology, the University of Texas Medical Branch at Galveston, Galveston, USA.
  6. 6Department of Biotechnology, Indian Academy Degree College-Autonomous, Bangalore, Karnataka, INDIA.
  7. 7Department of Haematology and blood transfusion, Padma Shree Institute of Medical Laboratory Technology, Rajiv Gandhi University of Health Sciences, Bangalore, Karnataka, INDIA.

CORRESPONDENCE

Mohammad Javad Mehran

Department of Biotechnology, JSS Research Foundation, SJCE Technical Campus, University of Mysore, Mysore-570006, Karnataka, INDIA.

mehran8150@gmail.com

ORCID: 0000-0001-7925-8239

Received: 11-06-2024; Revised: 07-10-2024; Accepted: 18-11-2024.

Volume 59, Issue 1 · pp. 196–209 · PUBLISHED Jan-Mar 2025 · DOI: 10.5530/ijper.20250081

View on IJPER original site ↗

ABSTRACT

Background Helicobacter pylori is implicated in several severe gastrointestinal disorders, including gastric cancer, affecting a significant global population. This study aims to exploit plant biotechnology for vaccine development by engineering brinjal (Solanum melongena L.) to express H. pylori’s cytotoxin-associated gene A (cagA) antigen. Utilizing transgenic plants as an innovative strategy not only mitigates the high costs associated with traditional vaccine production but also leverages their capacity to induce a mucosal immune response. Materials and Methods We used the brinjal variety ‘Arka Keshav’ for transformation. The cagA gene from H. pylori strain 26695 was cloned into the pBI121 vector and transferred into brinjal using Agrobacterium tumefaciens-mediated transformation. Transgenic expression was verified through PCR, quantitative real-time PCR (qPCR), Western blotting and ELISA. Immunohistochemistry was used to assess the localization of the cagA protein within the plant tissues. Results Cloning and amplification confirmed the insertion of the cagA gene approximately ~1700 bp in size. Transgenic brinjal lines were successfully generated, with distinct expression levels of cagA observed. ELISA and Western blot analyses indicated significant cagA protein expression, particularly in lines B11 and B17, which showed the highest antigen concentrations. The consistency of mRNA and protein expression validated the effectiveness of the transgenic approach. Conclusion The study demonstrates the feasibility of using genetically modified brinjal as a platform for producing edible vaccines against H. pylori. This approach not only presents a cost-effective alternative to traditional vaccines but also offers potential for enhancing accessibility in regions burdened by gastric diseases associated with H. pylori. Future research should focus on optimizing expression systems and evaluating the clinical efficacy of these plant-based vaccines.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Mehran, M. J., Barzigar, R., Kumar, B. Y. S., Haraprasad, N., Fakrudin, B., Paul, S., Malla, S., & Bolideei, M. (2025). Expression of Helicobacter pylori CagA in Solanum melongena L-A Cost Effective Strategy for Vaccine Development. Indian Journal of Pharmaceutical Education and Research, 59(1), 196–209. https://doi.org/10.5530/ijper.20250081