Development and Validation of a High-Performance Liquid Chromatographic Method for Content Assay of Ceftriaxone in Pharmaceutical Dosage
Basavaraj Hiremath★★ Corresponding author
- 1Department of Chemistry, S. S. Margol College of Arts, Science and Commerce, Shahabad, Kalaburagi, Karnataka, INDIA.
CORRESPONDENCE
Basavaraj Hiremath
Dr. BasavarajHiremath, Assistant Professor, Department of Chemistry, S. S. Margol College of Arts, Science and Commerce, Shahabad-585228, Kalaburagi, Karnataka, INDIA.
Received: 30-10-2023; Revised: 23-01-2024; Accepted: 19-10-2024.
Volume 59, Issue 1 · pp. 94–100 · PUBLISHED Jan-Mar 2025 · DOI: 10.5530/ijper.20255575
ABSTRACT
Aim A sensitive and accurate RP-HPLC method was developed and validated for the quantitative measurement of Ceftriaxone sodium (CEF) in pharmaceutical dosage forms and bulk medicine. Materials and Methods Ceftriaxone sodium was separated on a 245 nm photodiode array detector using a Waters XTerra RP-18 (5 μm 250x4.6 mm internal diameter) column. Results The method generated an excellent linear response in the concentration range of 0.2-20 μg/mL with remarkable precision of 0.16-0.7% and accuracy in the percent recovery range of 99.88-99.97%. Conclusion We find the reverse phase HPLC technique to be highly sensitive, accurate, precise and user-friendly and hence recommend it. Therefore, it could be useful in a quality control lab.
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Hiremath, B. (2025). Development and Validation of a High-Performance Liquid Chromatographic Method for Content Assay of Ceftriaxone in Pharmaceutical Dosage. Indian Journal of Pharmaceutical Education and Research, 59(1), 94–100. https://doi.org/10.5530/ijper.20255575