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Journal of Young PharmacistsNOW IN
Article

Spectrophotometric and Reversed-Phase High-Performance Liquid Chromatographic Method for the Determination of Doxophylline in Pharmaceutical Formulations

Joshi HR1, Patel AH2★, Captain AD3★ Corresponding author

  1. 1Shree Swaminarayan College of Pharmacy, Kalol Highway Road, Kalol, INDIA.
  2. 2Shree Swaminarayan College of Pharmacy, Kalol Highway Road, Kalol, INDIA.
  3. 3A.R. College of Pharmacy and Institute of Science and Technology for Advance Research (I.S.T.A.R), Vallabh Vidyanagar, Gujarat, INDIA.

CORRESPONDENCE

Patel AH

Shree Swaminarayan College of Pharmacy, Kalol Highway Road, Kalol, INDIA.

avnishhpatel@yahoo.co.in

Volume 2, Issue 3 · pp. 289–296 · PUBLISHED · DOI: 10.4103/0975-1483.66791

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ABSTRACT

Two methods are described for determination of Doxophylline in a solid dosage form. The first method was based on ultraviolet (UV)-spectrophotometric determination of the drug. It involves absorbance measurement at 274 nm (λmax of Doxophylline) in 0.1 N hydrochloric acid. The calibration curve was linear, with the correlation coefficient between 0.99 and 1.0 over a concentration range of 0.20–30 mg/ml for the drug. The second method was based on high-performance liquid chromatography (HPLC) separation of the drug in reverse-phase mode using the Hypersil ODS C18 column (250 X 4.6 mm, 5 mm). The mobile phase constituted of buffer acetonitrile (80:20) and pH adjusted to 3.0, with dilute orthophosphoric acid delivered at a flow rate 1.0 ml/min. Detection was performed at 210 nm. Separation was completed within 7 min. The calibration curve was linear, with the correlation coefficient between 0.99 and 1.0 over a concentration range of 0.165–30 mg/ml for the drug. The relative standard deviation was found to be <2.0% for the UV-spectrophotometry and HPLC methods. Both these methods have been successively applied to the solid dosage pharmaceutical formulation, and were fully validated according to ICH guidelines.

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    HR, J., AH, P., & AD, C. (). Spectrophotometric and Reversed-Phase High-Performance Liquid Chromatographic Method for the Determination of Doxophylline in Pharmaceutical Formulations. Journal of Young Pharmacists, 2(3), 289–296. https://doi.org/10.4103/0975-1483.66791