Effect of Arginine on IL-6, IL-17 and TGF-β levels in high-fat Diet-Induced Hypercholesterolemia Rat
Eryati Darwin1, Eka Fithra Elf2, Dachriyanus d3★★ Corresponding author
- 1Department of Histology and Immunology, Faculty of Medicine Andalas University, Padang, INDONESIA.
- 2Department of Cardiology, Faculty of Medicine Andalas University, Padang, INDONESIA.
- 3Faculty of Pharmacy Andalas University, Padang, INDONESIA.
CORRESPONDENCE
Dachriyanus d
Faculty of Pharmacy Andalas University, Padang, INDONESIA.
Received: 02-07-2016; Revised: 30-07-2016; Accepted: 02-09-2016.
Volume 9, Issue 1 · pp. 83–86 · PUBLISHED · DOI: 10.5530/jyp.2017.9.16
ABSTRACT
Objective: The aim with the present study was to determine that arginine affect to the level of IL-6, IL-17 and TGF-β in high-fat diet-induced hyper cholesterolemia in rats. Material and Methods: Male Wistar rats in eight weeks old (n=35) were divided into five groups, one group as a control (C) were given standard r fed and four groups were given high-fat diet (Hfd) with fats from beef tallow. One group of high-fat diet rats recieved no arginine, three groups were recieved arginine in doses of 100, 200 and 400 mg/kg/day each for three weeks. After two weeks of treatment, blood cholesterol were measured, while serum level of IL-6, IL-17 and TGF-β were measured at the final five weeks of this protocol by ELISA methods. Results:There were no significantly different between groups, which received different doses of arginine (p>0,05). IL-17 of high-fat diet received arginine of doses 100 and 200 mg/kg/day were significantly higher com pared to high-fat diet received no arginine and control group. Interestingly, IL-17 in high-fat diet group received arginine of doses 400 mg/kg/day were lower compared to groups received lower doses of arginine. TGF-β levels in the high-fat diet group’s recieved arginine were lower compared to control group. Conclusions:The effect of arginine on cytokines plays the role on vascular inflammation due to high-fat diet.
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Darwin, E., Elf, E. F., & d, D. (). Effect of Arginine on IL-6, IL-17 and TGF-β levels in high-fat Diet-Induced Hypercholesterolemia Rat. Journal of Young Pharmacists, 9(1), 83–86. https://doi.org/10.5530/jyp.2017.9.16