Chromatographic method for determination of the free amino acid content of chamomile flowers
Xiaoli Ma1, Dongsheng Zhao1, Xinxia Li1★, Lei Meng1★ Corresponding author
- 1Analytical and Testing Center, School of Pharmacy, Xinjiang Medical University, Urumqi, Xiangjiang, China.
CORRESPONDENCE
Xinxia Li
Analytical and Testing Center, School of Pharmacy, Xinjiang Medical University, Urumqi, Xiangjiang, China.
Received: 07-01-2013; Revised: 10-01-2013.
Volume 11, Issue 41 · pp. 176–179 · PUBLISHED 21 January 2015 · DOI: 10.4103/0973-1296.149735
ABSTRACT
Objective: To determine the free amino acid contents of chamomile flowers using reverse‑phase high‑performance column chromatography preceded by pre‑column derivatization with 6‑aminoquinolyl‑N‑hydroxysuccinimidyl carbamate (AQC), and to determine the reliability of this method. Materials and Methods: Derivatization with reconstituted AQC was used to prepare the samples and standards for injection into the chromatography column. The peaks were analyzed by fluorescence detection (λ excitation, 250 nm; λ emission, 395 nm. Results: Alanine, proline, and leucine were the most abundant amino acids, whereas tyrosine and methionine were the least abundant. The linearity of the method was found to be good with amino acid concentrations of 0.012-0.36 µM. The precision was 0.05-1.36%; average recovery, 91.12-129.41%; and limit of detection, 0.006-0.058 µM. Conclusion: The method is reliable for determining the free amino acid content of different types of chamomile flowers.
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Ma, X., Zhao, D., Li, X., & Meng, L. (2015). Chromatographic method for determination of the free amino acid content of chamomile flowers. Pharmacognosy Magazine, 11(41), 176–179. https://doi.org/10.4103/0973-1296.149735