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Article

Simultaneous Quantitative Determination of 12 Active Components in Yuanhu Zhitong Prescription by RP‑HPLC Coupled with Photodiode Array Detection

Xiaokai Zhang2,3, Song Zhang4, Qian Yang2,3, Wei Cao2,3, Yanhua Xie2,3, Pengcheng Qiu2,3, Siwang Wang2,3★★ Corresponding author

  1. 1Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi’an, China.
  2. 2The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains, Xi’an, China.
  3. 3Pharmacy Department, Tangdu Hospital, Fourth Military Medical University, Xi’an, People’s Republic of, China.

CORRESPONDENCE

Siwang Wang

The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains, Xi’an, China.

wangsiw@fmmu.edu.cn

Received: 13-02-2014; Revised: 25-04-2014.

Volume 11, Issue 41 · pp. 61–68 · PUBLISHED 21 January 2015 · DOI: 10.4103/0973-1296.149705

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ABSTRACT

Background: Yuanhu Zhitong prescription (YZP) is a famous traditional Chinese medicine formula, which is officially recorded in Chinese Pharmacopoeia for the treatment of stomach pain, hypochondriac pain, headache and dysmenorrhea caused by qi‑stagnancy and blood stasis. It is the first report for the simultaneous determination of 12 active components in YZP. Objective: A newly, simple, accurate and reliable method for the separation and determination of 12 active components (protopine, α‑allocryptopine, coptisine, xanthotol, palmatine, dehydrocorydaline, glaucine, tetrahydropalmatine, tetrahydroberberine, imperatorin, corydaline, isoimperatorin) in YZP was developed and validated using HPLC‑PAD. Materials and Methods: The analytes were performed on a Phenomenex Luna‑C18 (2) column (250×4.6 mm, 5.0 μm) with a gradient elution program using a mixture of acetonitrile and 0.1% phosphoric acid water solution (adjusted with triethylamine to pH 5.6) as mobile phase. Analytes were performed at 30°C with a flow rate of 1.0 mL/min. Results: The validated method was applied to analyze four major dosage forms of YZP coming from different manufacturers with good linearity (r2, 0.9981~0.9999), precision (RSD, 0.24~2.89%), repeatability (RSD, 0.15~3.34%), stability (RSD, 0.14~3.35%), recovery (91.13~110.81%) of the 12 components. Conclusion: The proposed method enables the separation and determination of 12 active components in a single run for the quality control of YZP.

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    Zhang, X., Zhang, S., Yang, Q., Cao, W., Xie, Y., Qiu, P., & Wang, S. (2015). Simultaneous Quantitative Determination of 12 Active Components in Yuanhu Zhitong Prescription by RP‑HPLC Coupled with Photodiode Array Detection. Pharmacognosy Magazine, 11(41), 61–68. https://doi.org/10.4103/0973-1296.149705