jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Pharmacognosy MagazineNOW IN
Article

Preparation of Ginseng Extract with Enhanced Levels of Ginsenosides Rg1 and Rb1 Using High Hydrostatic Pressure and Polysaccharide Hydrolases

Sasikumar Arunachalam Palaniyandi1,2, Joo-Won Suh2,3★, Seung Hwan Yang4★★ Corresponding author

  1. 1Interdisciplinary Program of Biomodulation, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, Korea.
  2. 2Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, Korea.
  3. 3Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, Korea.
  4. 4Department of Biotechnology, Chonnam National University, Daehak-ro, Yeosu-si, Jeollanam-do, Korea.

CORRESPONDENCE

Joo-Won Suh

Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, Korea.

ymichigan@jnu.ac.kr

Received: 22-09-2015; Revised: 12-11-2015; Accepted: 12-11-2015.

Volume 13, Issue 49S · pp. 142–7 · PUBLISHED 7 April 2017 · DOI: 10.4103/0973-1296.203992

View on PM original site ↗

ABSTRACT

Background: Ginsenosides are the principal components responsible for the pharmacological activities of ginseng. Ginsenosides Rg1 and Rb1 are the major compounds recognized as marker substances for quality control of ginseng-based products. These major compounds can be transformed to several pharmacologically active minor ginsenosides by chemical, microbial, and enzymatic means. Materials and Methods: In the present study, a combination of polysaccharide hydrolases and high hydrostatic pressure (HHP) were used to extract ginseng saponins enriched with ginsenosides Rg1 and Rb1. Temperature, pH, time, ginseng-to-water ratio, and pressure were optimized to obtain the maximum amount of Rg1 and Rb1 in the resulting extract using commercial polysaccharide hydrolases. Results: This study showed that treatment with a combination of cellulase, amylase, and pectinase at 100 MPa pressure, pH 4.8, and 45°C for 12 h resulted in higher Rg1 and Rb1 levels in the extract. Conclusion: This study describes a cheap and ecofriendly method for preparing ginseng extract enriched with Rg1 and Rb1.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Palaniyandi, S. A., Suh, J., & Yang, S. H. (2017). Preparation of Ginseng Extract with Enhanced Levels of Ginsenosides Rg1 and Rb1 Using High Hydrostatic Pressure and Polysaccharide Hydrolases. Pharmacognosy Magazine, 13(49S), 142–7. https://doi.org/10.4103/0973-1296.203992