In vitro Inhibitory Effects of Andrographis paniculata, Gynura procumbens, Ficus deltoidea, and Curcuma xanthorrhiza Extracts and Constituents on Human Liver Glucuronidation Activity
Zulhilmi Husni1, Sabariah Ismail1★, Mohd Halimhilmi Zulkiffli1, Atiqah Afandi1, Munirah Haron1★ Corresponding author
- 1Centre for Drug Research, Universiti Sains Malaysia, Pulau Pinang, Malaysia.
CORRESPONDENCE
Sabariah Ismail
Centre for Drug Research, Universiti Sains Malaysia, Pulau Pinang, Malaysia.
Received: 21-06-2016; Revised: 22-07-2016.
Volume 13, Issue 50S · pp. S236–S243 · PUBLISHED 11 July 2017 · DOI: 10.4103/pm.pm_299_16
ABSTRACT
Background: Andrographis paniculata, Gynura procumbens, Ficus deltoidea and Curcuma xanthorrhiza are commonly consumed as herbal medicines. However their effects on human liver glucuronidation activity are not yet evaluated. Objective: In this study, we evaluate the inhibitory Effects of Andrographis paniculata, Gynura procumbens, Ficus deltoidea and Curcuma xanthorrhiza extracts and their constituents on human liver glucuronidation activity. Materials and Methods: Herbal extracts (aqueous, methanolic and ethanolic extracts) and their constituents were incubated with human liver microsomes with the addition of UDPGA to initiate the reaction. Working concentrations of herbal extracts and their constituents ranged from 10 µg/mL to 1000 µg/mL and 10 µM to 300 µM respectively. IC50 was determined by monitoring the decrement of glucuronidation activity with the increment of herbal extracts or phytochemical constituent’s concentrations. Results: All herbal extracts inhibited human liver glucuronidation activity in range of 34.69 µg/mL to 398.10 µg/mL whereas for the constituents, only xanthorrhizol and curcumin (constituents of Curcuma xanthorrhiza) inhibited human liver glucuronidation activity with IC50 of 538.50 and 32.26 µM respectively. Conclusion: In the present study, we have proved the capabilities of Andrographis paniculata, Gynura procumbens, Ficus deltoidea and Curcuma xanthorrhiza to interfere with in vitro glucuronidation process in human liver microsomes.
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Husni, Z., Ismail, S., Zulkiffli, M. H., Afandi, A., & Haron, M. (2017). In vitro Inhibitory Effects of Andrographis paniculata, Gynura procumbens, Ficus deltoidea, and Curcuma xanthorrhiza Extracts and Constituents on Human Liver Glucuronidation Activity. Pharmacognosy Magazine, 13(50S), S236–S243. https://doi.org/10.4103/pm.pm_299_16