jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Pharmacognosy MagazineNOW IN
Article

Caesalpinia Crista Linn. Induces Protection against DNA and Membrane Damage

R. Sunil Kumar1, Ramesh Balenahalli Narasingappa1★, Chandrashekhar G. Joshi2, Talakatta K. Girish3, Ananda Danagoudar2★ Corresponding author

  1. 1Department of Biotechnology, College of Agriculture, University of Agriculture Sciences, Bangalore, Hassan, India.
  2. 2Department of Studies and Research in Biochemistry, P.G Centre, Mangalore University, Chikka Aluvara, Kodagu, India.
  3. 3Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, Karnataka, India.

CORRESPONDENCE

Ramesh Balenahalli Narasingappa

Department of Biotechnology, College of Agriculture, University of Agriculture Sciences, Bangalore, Hassan, India.

rameshbn20@uasbangalore.edu.in

Received: 09-12-2016; Revised: 11-01-2017.

Volume 13, Issue 50S · pp. S250–S257 · PUBLISHED 11 July 2017 · DOI: 10.4103/pm.pm_557_16

View on PM original site ↗

ABSTRACT

Background: Caesalpinia crista is a medicinal herb used to cure various ailments in subtropical and tropical regions of Southeast Asia. Objective: The objective of this evaluation of C. crista against free radical induced DNA and erythrocyte damage. Materials and Methods: The profiles of polyphenol and flavonoid were quantified through reversed‑phase high‑performance liquid chromatography. Free radical induced DNA and membrane damage were performed using H2O2 as oxidative agent. Results: The total polyphenol content of C. crista leaf ethyl acetate extract (CcEA) was 94.5 ± 3.8 mg/ gGAE, CcME (C. crista leaf methanol extract) was 52.7 ± 2.8 mg/gGAE, and CcWE (C. crista leaf Water extract) was 31.84 ± 1.8 mg/gGAE. Total flavonoid content of CcEA was 60.46 ± 2.3 mg/gQE, CcME was 46.26 ± 1.8 mg/ gQE, and CcWE was 20.47 ± 1.1 mg/gQE. The extracts also exhibited good antioxidant activity as confirmed by 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH), 2,2’‑azino‑bis (3‑ethylbenzthiazoline‑6‑sulfonic acid), hydroxyl scavenging, reducing power, and total antioxidant assays. Among the three extracts, CcEA and CcME showed better protection against red blood cell (RBC) hemolysis and DNA damage as confirmed by electrophoretic study. Further, Scanning electron micrograph data showed that CcEA revealed the free radical induced structural alterations in RBC. Conclusion: These findings suggest that C. crista contains bioactive molecules and can inhibit oxidative stress and can be source of further study to use this in herbal medicine.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Kumar, R. S., Narasingappa, R. B., Joshi, C. G., Girish, T. K., & Danagoudar, A. (2017). Caesalpinia Crista Linn. Induces Protection against DNA and Membrane Damage. Pharmacognosy Magazine, 13(50S), S250–S257. https://doi.org/10.4103/pm.pm_557_16