Simultaneous Determination of Four Compounds, Campesterol, Emodin8‑O‑β‑D‑Glucopyranoside, Quercetin, and Isoquercitrin in Reynoutria sachalinensis by High‑performance Liquid Chromatography‑Diode Array Detector
Min Rye Eom2, Jin Bae Weon2, Youn Sik Jung2, Ga Hee Ryu2, Woo Seung Yang2, Choong Je Ma2,3★★ Corresponding author
- 1Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University.
- 2Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200‑701, Republic of Korea.
CORRESPONDENCE
Choong Je Ma
Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200‑701, Republic of Korea.
Received: 15-06-2016; Revised: 01-09-2016.
Volume 13, Issue 50S · pp. S258–S261 · PUBLISHED 11 July 2017 · DOI: 10.4103/pm.pm_289_16
ABSTRACT
Background: Reynoutria sachalinensis is a well‑known and used herbal medicine to treatment of arthralgia, jaundice, amenorrhea, coughs, carbuncles, and sores. Objective: We have developed high‑performance liquid chromatography analysis method for simultaneous determination of isolated four compounds, campesterol, emodin8‑O‑β‑D‑glucopyranoside, quercetin, and isoquercitrin from R. sachalinensis is. Materials and Methods: The four compounds were separated on Shiseido C18 column (S‑5 µm, 4.6 mm I.D. ×250 mm) at a column temperature of 25°C. The mobile phase composed of water and methanol with gradient elution system, and flow rate is 1.0 ml/min. The detection wavelength was set at 205 nm. Results: Validation of this analytical method was evaluated by linearity, precision, and accuracy test. This established method had good linearity (R2 > 0.997). The relative standard deviation values of intra‑ and inter‑day testing were indicated that <2%, and accuracy is 91.66%–103.31% at intraday and 91.69%– 103.31% at intraday. The results of recovery test were 92.60%–108.99%. Conclusion: In these results, developed method was accurate and reliable to the quality evaluation of campesterol, emodin 8‑O‑β‑D‑glucopyranoside, quercetin, and isoquercitrin isolated from R. sachalinensis.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Eom, M. R., Weon, J. B., Jung, Y. S., Ryu, G. H., Yang, W. S., & Ma, C. J. (2017). Simultaneous Determination of Four Compounds, Campesterol, Emodin8‑O‑β‑D‑Glucopyranoside, Quercetin, and Isoquercitrin in Reynoutria sachalinensis by High‑performance Liquid Chromatography‑Diode Array Detector. Pharmacognosy Magazine, 13(50S), S258–S261. https://doi.org/10.4103/pm.pm_289_16