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Article

Peimine Inhibits the Production of Proinflammatory Cytokines Through Regulation of the Phosphorylation of NF-κB and MAPKs in HMC-1 Cells

Ji Hye Park1, Bina Lee1, Hyun Kab Kim1, Eun-Young Kim1, Jae-Hyun Kim1, Ju-Hee Min1, Sunkook Kim2, Youngjoo Sohn1, Hyuk-Sang Jung1★★ Corresponding author

  1. 1Department of Anatomy, College of Korean Medicine, Kyung Hee University, Kyungheedae-ro, Dongdaemun-gu, Seoul, Korea.
  2. 2Multi-Functional Nano/Bio Electronics Laboratory, Kyung Hee University, Gyeonggi-do, Korea.

CORRESPONDENCE

Hyuk-Sang Jung

Department of Anatomy, College of Korean Medicine, Kyung Hee University, Kyungheedae-ro, Dongdaemun-gu, Seoul, Korea.

jhs@khu.ac.kr

Received: 12-08-2016; Revised: 24-10-2016; Accepted: 24-10-2016.

Volume 13, Issue 50S · pp. S359–64 · PUBLISHED 11 July 2017 · DOI: 10.4103/0973-1296.210173

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ABSTRACT

Background: Peimine is a major biologically active component of Fritillaria ussuriensis. Peimine was investigated in chronic inflammation response, but it has not been studied in mast cell-related immediate allergic reaction. The present study aimed to evaluate anti-allergic effect of peimine in human mast cell (HMC-1). Materials and Methods: The effect of peimine on cell viability was measured by MTS assay in HMC-1. Histamine release was investigated in rat peritoneal mast cells (RPMCs). Interleukin (IL)-6, IL-8, and tumor necrosis factor-α (TNF-α) expressions were measured by ELISA assay and reverse transcription-polymerase chain reaction. Mitogen-activated protein kinases (MAPKs) and nuclear factor-kappaB (NF-κB) were examined by Western blot. Passive cutaneous anaphylaxis (PCA) reactions were evaluated using Sprague-Dawley (SD) rats. Results: Peimine inhibited the production of pro-inflammatory cytokines, such as IL-6, IL-8, and TNF-α. Moreover, peimine reduced MAPKs phosphorylation and the nuclear NF-κB expression in PMACI-induced HMC-1. Peimine decreased PCA reactions in rats as well. Conclusion: Our study proved that peimine might be suitable for the treatment of mast cell-derived allergic inflammatory reactions.

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    Park, J. H., Lee, B., Kim, H. K., Kim, E., Kim, J., Min, J., Kim, S., Sohn, Y., & Jung, H. (2017). Peimine Inhibits the Production of Proinflammatory Cytokines Through Regulation of the Phosphorylation of NF-κB and MAPKs in HMC-1 Cells. Pharmacognosy Magazine, 13(50S), S359–64. https://doi.org/10.4103/0973-1296.210173