jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Pharmacognosy MagazineNOW IN
Article

In vitro Cell Viability by CellProfiler® Software as Equivalent to MTT Assay

Luciana S Gasparini1, Nayana D Macedo1, Elisângela F Pimentel1, Marcio Fronza1, Valdemar L Junior2, Warley S Borges2, Eduardo R Cole1, Tadeu U Andrade1, Denise C Endringer1, Dominik Lenz1★★ Corresponding author

  1. 1University Vila Velha, Pharmaceutical Sciences, Vitória, Brazil.
  2. 2Federal University of Espírito Santo, Vitória, Brazil.

CORRESPONDENCE

Dominik Lenz

University Vila Velha, Pharmaceutical Sciences, Vitória, Brazil.

dominic.lenz@uvv.br

Received: 12-09-2016; Revised: 08-11-2016; Accepted: 08-11-2016.

Volume 13, Issue 50S · pp. S365–9 · PUBLISHED 11 July 2017 · DOI: 10.4103/0973-1296.210176

View on PM original site ↗

ABSTRACT

Objective: This study evaluated in vitro cell viability by the colorimetric MTT stands for 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay compared to image analysis by CellProfiler® software. Materials and Methods: Hepatoma (Hepa-1c1c7) and fibroblast (L929) cells were exposed to isolated substances, camptothecin, lycorine, tazettine, albomaculine, 3-epimacronine, trispheridine, galanthine and Padina gymnospora, Sargassum sp. methanolic extract, and Habranthus itaobinus Ravenna ethyl acetate in different concentrations. After MTT assay, cells were stained with Panotic dye kit. Cell images were obtained with an inverted microscope equipped with a digital camera. The images were analyzed by CellProfiler®. Results: No cytotoxicity at the highest concentration analyzed for 3-epimacronine, albomaculine, galanthine, trispheridine, P. gymnospora extract and Sargassum sp. extract where detected. Tazettine offered cytotoxicity only against the Hepa1c1c7 cell line. Lycorine, camptothecin, and H. itaobinus extract exhibited cytotoxic effects in both cell lines. The viability methods tested were correlated demonstrated by Bland–Atman test with normal distribution with mean difference between the two methods close to zero, bias value 3.0263. The error was within the limits of the confidence intervals and these values had a narrow difference. The correlation between the two methods was demonstrated by the linear regression plotted as R2. Conclusion: CellProfiler® image analysis presented similar results to the MTT assay in the identification of viable cells, and image analysis may assist part of biological analysis procedures. The presented methodology is inexpensive and reproducible.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Gasparini, L. S., Macedo, N. D., Pimentel, E. F., Fronza, M., Junior, V. L., Borges, W. S., Cole, E. R., Andrade, T. U., Endringer, D. C., & Lenz, D. (2017). In vitro Cell Viability by CellProfiler® Software as Equivalent to MTT Assay. Pharmacognosy Magazine, 13(50S), S365–9. https://doi.org/10.4103/0973-1296.210176