Orthogonal test design for optimizing the extraction of total flavonoids from Inula helenium
Chun-Yan Guo2★, Jin Wang3★, Yong Hou3, Yong-Ming Zhao3, Li-Xia Shen3, Dan-Shen Zhang2,4★★ Corresponding author
- 1Department of Graduate, HeBei Medical University, Shijiazhuang 050017, Hebei, China.
- 2Department of Pharmacy, HeBei North University, Zhangjiakou, 075000, China.
- 3HeBei University of Science and Technology, Shijiazhuang, 050018, China.
CORRESPONDENCE
Chun-Yan Guo
Department of Pharmacy, HeBei North University, Zhangjiakou, 075000, China.
Received: 02-06-2012; Revised: 08-08-2012.
Volume 9, Issue 35 · pp. 166–170 · PUBLISHED 11 June 2013 · DOI: 10.4103/0973-1296.113260
ABSTRACT
Background: Inula helenium, which belongs to thecomposite family, is an important crude drug in traditional Chinese medicine. Materials and Methods: The effects of ethanol concentration, liquid to solid ratio, extraction temperature, and duration of microwave irradiation on the flavonoid extraction yield were studied through a single-factor experiment. An orthogonal array (L9(34)) was then constructed to achieve the best extraction conditions. Results: Variance analysis revealed that ethanol concentration significantly affected the extraction yield. The optimal conditions were as follows: ethanol concentration, 50% (v/v); liquid to solid ratio, 15:1; duration of microwave irradiation, 240 s; and extraction temperature, 60°C. Conclusion: Under these optimal conditions, the total flavonoid yield was 18.34 ± 0.64 mg/g. The use of a microwave-assisted process dramatically reduced the time needed for extraction of flavonoids from I. helenium.
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Guo, C., Wang, J., Hou, Y., Zhao, Y., Shen, L., & Zhang, D. (2013). Orthogonal test design for optimizing the extraction of total flavonoids from Inula helenium. Pharmacognosy Magazine, 9(35), 166–170. https://doi.org/10.4103/0973-1296.113260