Anti-quorum sensing potential of Adenanthera pavonina
Halkare Suryanarayana Vasavi1, Ananthapadmanabha Bhagwath Arun1, Punchappady-Devasya Rekha1★★ Corresponding author
- 1Yenepoya Research Center, Yenepoya University, University Road, Deralakatte, Mangalore, Karnataka, India
CORRESPONDENCE
Punchappady-Devasya Rekha
*Address for correspondence: Dr. P. D. Rekha, Deputy Director, Yenepoya Research Centre Yenepoya University, University Road, Deralakatte, Mangalore - 575 018, Karnataka, India.
Revised: 26-04-2014.
Volume 7, Issue 1 · pp. 105–109 · PUBLISHED January-March 2015 · DOI: 10.4103/0974-8490.147220
ABSTRACT
Background Quorum sensing (QS) in Pseudomonas aeruginosa plays a key role in virulence factor production, biofilm formation and antimicrobial resistance. Because of emerging antimicrobial resistance in P. aeruginosa, there is a need to find an alternate nonantibiotic agent for the control of infections caused by this organism. Objective: To evaluate anti-QS activity of Adenanthera pavonina L., a medicinal plant used in traditional medicine. Materials and Methods Preliminary screening for anti-QS activity of ethanol extract of A. pavonina was carried out using Chromobacterium violaceum CV026 biosensor strain and inhibition of QS-regulated violacein production was quantified using C. violaceum ATCC12472. Bioassay guided fractionation of ethanol extract resulted in ethyl acetate fraction (AEF) with strong anti-QS activity and AEF was evaluated for inhibition of QS-regulated pyocyanin production, proteolytic, elastolytic activity, swarming motility and biofilm formation in P. aeruginosaPAO1. Results AEF, at 0.5 mg/ml, inhibited pyocyanin production completely and at 1 mg/ml of AEF, complete inhibition of proteolytic and elastolytic activities were observed. However, viability of P. aeruginosa PAO1 was not affected at the tested concentrations of AEF as observed by cell count. Swarming motility was inhibited at the concentration of 0.1 mg/ml of AEF. Thin layer chromatography and biosensor overlay of AEF showed violacein inhibition zone at Rf value 0.63. Conclusion From the results of this study, it can be concluded that A. pavonina extracts can be used as effective anti-QS agents.
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Vasavi, H. S., Arun, A. B., & Rekha, P. (2015). Anti-quorum sensing potential of Adenanthera pavonina. Pharmacognosy Research, 7(1), 105–109. https://doi.org/10.4103/0974-8490.147220