HPTLC Fingerprinting: A Tool for Simplified Analysis of Phenolics in Medicinal Plants
Amit Yadaorao Saraf1★, Aparna Amit Saraf2★ Corresponding author
- 1Department of Botany, Government of Maharashtra’s Ismail Yusuf College, Jogeshwari (E), Mumbai, Maharashtra INDIA.
- 2Department of Botany, Dr Homi Bhabha State University, The Institute of Science 15 Madame, Mumbai, Maharashtra INDIA.
CORRESPONDENCE
Amit Yadaorao Saraf
Department of Botany, Government of Maharashtra’s Ismail Yusuf College, Jogeshwari (E), Mumbai, Maharashtra INDIA.
Received: 18-04-2020; Revised: 17-07-2020; Accepted: 23-10-2020.
Volume 54, Issue 4 · pp. 1098–1103 · PUBLISHED 2020 · DOI: 10.5530/ijper.54.4.205
ABSTRACT
Background: Phenolic compounds especially the flavonoids and tannins comprise one of the most significant classes of plant secondary metabolites. Aim: HPTLC is widely accepted by WHO and pharmacopoeia across the globe as an effective analytical method for the investigation of phenolics in herbal analyses. Materials and Methods: The methanolic extracts of Abrus precatorius L., Sapindus trifoliatus L. and Embelia ribes Burm. F. were developed on the HPTLC system to study the diversity of phenolic compounds like flavonoids and tannins under different development conditions. Flavanoids were separated using the combination of solvent system comprising of mid-polar to polar solvents, hence Ethyl acetate: formic acid: glacial acetic acid: water (10:0.5:0.5:1 v/v/v/v) solvent mixture and 10% methanolic Sulphuric Acid Reagent was used as spray reagent for separation of flavanoids. Tannins were separated on chromatogram by using the solvent system comprising of mid-polar to non-polar solvents, hence Toluene: Ethyl Acetate: Formic Acid (6:4:0.3 v/v/v) as solvent system and alcoholic FeCl3 was used for derivatization. Results: Distinct fingerprint of flavanoids and tannins was obtained. A profile of 11 to 12 polyvalent phytoconstituents were separated during the HPTLC analysis of flavonoids and tannins respectively. Conclusion: The HPTLC analysis successfully demonstrated that phenolic secondary metabolites can be effectively separated using the same extract under different development conditions. The detection and profiling of such metabolites provides the justification for therapeutic activities in medicinal plants.
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Saraf, A. Y., & Saraf, A. A. (2020). HPTLC Fingerprinting: A Tool for Simplified Analysis of Phenolics in Medicinal Plants. Indian Journal of Pharmaceutical Education and Research, 54(4), 1098–1103. https://doi.org/10.5530/ijper.54.4.205