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Article

Simultaneous determination of four bioactive compounds in Glechoma longituba extracts by high performance liquid chromatography

Qiyuan Shan2,3, Gang Cao2,3, Hao Cai2★, Baochang Cai2,3★ Corresponding author

  1. 1Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, China.
  2. 2Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou, P. R, China.

CORRESPONDENCE

Hao Cai

Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou, P. R, China.

haocai_98@126.com

Received: 03-04-2012; Revised: 29-04-2012.

Volume 9, Issue 35 · pp. 216–219 · PUBLISHED 11 June 2013 · DOI: 10.4103/0973-1296.113269

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ABSTRACT

Background: Glechoma longituba, one of the long-practiced traditional Chinese medicines in history, is still commonly used nowadays in oriental countries. Previous study indicates that phenolic acid and flavonoids have considerable bioactivities, thus, chlorogenic acid, caffeic acid, apigenin-7- O-glucoside and rosmarinic acid were chosen as the marker components for the simultaneous determination to evaluate the intrinsic quality of G. longituba, and related high performance liquid chromatographic method was urgent to established. Materials and Methods: A HPLC method was established for simultaneous determination of four major active components in G. longituba, three batches which collected from different suppliers in Zhejiang, Anhui and Jiangsu Provinces were determined. Results: The contents of chlorogenic acid, caffeic acid, apigenin-7-O-glucoside and rosmarinic acid in G. longituba samples analyzed were 0.00225-0.00234%, 0.0238-0.0242%, 0.00271-0.00313%, 0.830-0.896%, respectively. Conclusion: The developed method can be applied to the intrinsic quality control of G. longituba.

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    Shan, Q., Cao, G., Cai, H., & Cai, B. (2013). Simultaneous determination of four bioactive compounds in Glechoma longituba extracts by high performance liquid chromatography. Pharmacognosy Magazine, 9(35), 216–219. https://doi.org/10.4103/0973-1296.113269