A Simple RP-HPLC Method for Quantitation of Itopride HCl in Tablet Dosage Form
Thiruvengada Rajan VS1★, Mohamed Saleem TS2, Ramkanth S3, Alagusundaram M3, Ganaprakash K3, Madhusudhana Chetty 33★ Corresponding author
- 1Departments of Pharmaceutical Analysis, INDIA.
- 2Pharmacology, INDIA.
- 3Pharmaceutics, Annamacharya College of Pharmacy, Rajampet, INDIA.
CORRESPONDENCE
Thiruvengada Rajan VS
Departments of Pharmaceutical Analysis, INDIA.
Volume 2, Issue 4 · pp. 410–413 · PUBLISHED · DOI: 10.4103/0975-1483.71634
ABSTRACT
An isocratic reversed phase high-performance liquid chromatographic method with ultraviolet detection at 220 nm has been developed for the quantification of itopride hydrochloride in tablet dosage form. The quantification was carried out using C8 column (250 mm × 4.6 mm), 5-μm particle size SS column. The mobile phase comprised of two solvents (Solvent A: buffer 1.4 mL ortho-phosphoric acid adjusted to pH 3.0 with triethyl amine and Solvent B: acetonitrile). The ratio of Solvent A: Solvent B was 75:25 v/v. The flow rate was 1.0 mL-1 with UV detection at 220 nm. The method has been validated and proved to be robust. The calibration curve was linear in the concentration range of 80-120% with coefficient of correlation 0.9995. The percentage recovery for itopride HCl was 100.01%. The proposed method was validated for its selectivity, linearity, accuracy, and precision. The method was found to be suitable for the quality control of itopride HCl in tablet dosage formulation.
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VS, T. R., TS, M. S., S, R., M, A., K, G., & 3, M. C. (). A Simple RP-HPLC Method for Quantitation of Itopride HCl in Tablet Dosage Form. Journal of Young Pharmacists, 2(4), 410–413. https://doi.org/10.4103/0975-1483.71634